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The complexes were concentrated by a molecular weight cut-off concentrator and separated by SEC on a Superose 6 Increase 10/300GL column with running buffer containing 20 mM HEPES, pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG, 0.002% (w/v) CHS and 5 μM peptide 20
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