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In vitro cell therapy also indicated that infusion of Treg cells could increase the expression of anti-inflammatory factors (such as IL-10, IL-4, TGF-β, IL-2), while reducing the levels of pro-inflammatory factors (such as IL-17, IFN-γ) ( 5.3 The "Tregs-Microglia-Neuron/NPCs" axis and GLP-1R/cAMP/PKA/IL-10 mediated immunosuppressive and neuroprotective effects of GLP-1RAs in ischemic stroke In summary, in the pathological state of ischemic stroke, T cells preferentially migrated to the lesion boundary, and significantly increased after ischemia, peaking in the following weeks and persisting for several months ( + cytotoxic T cells are the first subpopulation of T cells that invade the ischemic brain within a few hours after stroke ( Figure 3 ) ( Further studies showed that different subtypes of T cells had apparently different effects on microglia